The objective of this book is to provide detailed information to the principles of biophysical chemistry that gives the reads an insight into its concepts. The book delivers the fundamental principles of biochemistry and chemistry both. This book introduces about biopolymers and various types of biopolymer interactions and forces involved in biological systems. This book explains bioenergetics, free energy and binding energy concept used in formation of various biomolecular bonds and thermodynamic processes of living cells. The various biological phenomenon have been expressed from the view of a physicist. Various analytical techniques which are used in biological sciences, such as spectroscopy, X-ray spectroscopy, Electrophoresis, Chromatography, Optical Rotatary Dispersion, Circular Dichroism etc. have been discussed. The chapters provide a detailed and thorough overview of the topic and features problems and excersises throughout. At the end of each chapter a relative sum up of chapter is discussed. The comprehensive approach of the book will be suitable for students of B.Sc., M.Sc., M.Phil, Ph.D and also for preparing various competitive examination of equivalent standard. The book shall be used in coursework of general chemistry, biochemistry and polymer chemistry. This book contains information obtained from authentic and highly regarded sources.